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    Addgene inc aav9 synapsin promoted gcamp virus
    Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average <t>GCAMP</t> fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).
    Aav9 Synapsin Promoted Gcamp Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav9+synapsin+promoted+gcamp+virus/pAAV%2ESyn%2EGCaMP6f%2EWPRE%2ESV40+(Plasmid+%23100837)/bio_rxiv__2025__08__19__671076-156-21-26
    Average 97 stars, based on 224 article reviews
    aav9 synapsin promoted gcamp virus - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "Mechanistic basis of dynamic and heterogeneous divisive normalization in visual cortex"

    Article Title: Mechanistic basis of dynamic and heterogeneous divisive normalization in visual cortex

    Journal: bioRxiv

    doi: 10.1101/2025.08.19.671076

    Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average GCAMP fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).
    Figure Legend Snippet: Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average GCAMP fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).

    Techniques Used: Imaging, Fluorescence, Expressing, Activity Assay

    Related Articles

    Injection:

    Article Title: Mechanistic basis of dynamic and heterogeneous divisive normalization in visual cortex
    Article Snippet: .. At each injection site we lowered a glass-tipped syringe controlled by a microsyringe pump into the brain and injected 200nL of AAV9 synapsin-promoted GCaMP virus (pAAV.Syn.GCaMP6f.WPRE.SV40, Addgene product #100837 for GCaMP6f mice, pGP-AAV-syn-jGCaMP8s-WPRE, Addgene product #162374 for GCaMP8s mice) at two depths, roughly 250 and 500 microns below pia. ..

    Virus:

    Article Title: Mechanistic basis of dynamic and heterogeneous divisive normalization in visual cortex
    Article Snippet: .. At each injection site we lowered a glass-tipped syringe controlled by a microsyringe pump into the brain and injected 200nL of AAV9 synapsin-promoted GCaMP virus (pAAV.Syn.GCaMP6f.WPRE.SV40, Addgene product #100837 for GCaMP6f mice, pGP-AAV-syn-jGCaMP8s-WPRE, Addgene product #162374 for GCaMP8s mice) at two depths, roughly 250 and 500 microns below pia. ..



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    aav9 synapsin promoted gcamp virus  (Addgene inc)
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    Addgene inc aav9 synapsin promoted gcamp virus
    Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average <t>GCAMP</t> fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).
    Aav9 Synapsin Promoted Gcamp Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav9+synapsin+promoted+gcamp+virus/pAAV%2ESyn%2EGCaMP6f%2EWPRE%2ESV40+(Plasmid+%23100837)/bio_rxiv__2025__08__19__671076-156-21-26
    Average 97 stars, based on 1 article reviews
    aav9 synapsin promoted gcamp virus - by Bioz Stars, 2026-09
    97/100 stars
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    Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average GCAMP fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).

    Journal: bioRxiv

    Article Title: Mechanistic basis of dynamic and heterogeneous divisive normalization in visual cortex

    doi: 10.1101/2025.08.19.671076

    Figure Lengend Snippet: Experimental setup and basic description of normalization in mouse V1. (A) : Example imaging field of view in superficial mouse V1, with average GCAMP fluorescence in white and tdTomato expression in magenta. Colocalization of white and magenta indicates PV interneurons expressing GCaMP. (B): Example visual stimuli: large static sine-wave gratings at 50% contrast and the corresponding 100% contrast plaid resultant from summing two orthogonal gratings. (C): Example orientation tuning curves and responses to plaids at the preferred orientation for two recorded neurons, one with a positive normalization index (NI) corresponding to cross-orientation suppression, and one with a negative NI corresponding to facilitation. (D): Distribution of normalization indices (NI) for PV (N=78) and putative excitatory (N=1,325) cells pooled across mice, computed from the inferred spiking activity across the whole stimulus presentation. The median values for PV and E NI distributions, 0.23 and 0.31 respectively (indicated by dashed vertical lines), are not significantly different ( p = 0.222, Wilcoxon rank-sum test).

    Article Snippet: At each injection site we lowered a glass-tipped syringe controlled by a microsyringe pump into the brain and injected 200nL of AAV9 synapsin-promoted GCaMP virus (pAAV.Syn.GCaMP6f.WPRE.SV40, Addgene product #100837 for GCaMP6f mice, pGP-AAV-syn-jGCaMP8s-WPRE, Addgene product #162374 for GCaMP8s mice) at two depths, roughly 250 and 500 microns below pia.

    Techniques: Imaging, Fluorescence, Expressing, Activity Assay